The WUSCHELa (PtoWUSa) is involved in developmental plasticity of adventitious root in Poplar

作者: 时间:2020-04-10 点击数:

作者:Jianbo Li 1,2, Huixia Jia 2,3, Pei Sun 2,3, Jin Zhang 4, Yongxiu Xia 1, Jianjun Hu 2,3, Lijuan Wang 2,3*, and Mengzhu Lu 5,*

1.Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 102300, China; 2.State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China; 3.Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China; 4.Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA; 5.College of Forestry and Biotechnology, Zhejiang Agriculture & Forestry University, Hangzhou 311300, China.

期刊Genes 2020, 11, 176; doi:10.3390/genes11020176

基金:National Key Program on Transgenic Research (2018ZX08020002), the National Natural Science Foundation of China (31800569), and Fundamental Research Funds of Chinese Academy of Forestry (CAFYBB2019ZY003).

摘要:WUSCHEL-RELATED HOMEOBOX (WOX) transcription factors play critical roles in cell fate determination during plant development. As the founding member of the WOX family, WUSCHEL (WUS) is characterized for its role in maintaining stem cell in meristem. In this study, we investigated the function of Populus tomentosa WUSCHELa (PtoWUSa) in adventitious roots (ARs) in poplar. Expression profile analysis showed that PtoWUSa was not only expressed in shoot apical meristem and stem, but also expressed in ARs. Ectopic expression of PtoWUSa in Arabidopsis resulted in shortened primary root, as well as agravitropism and multiple branches. Overexpression of PtoWUSa in poplar increased the number of ARs but decreased their length. Moreover, the AR tip and lateral root tip became larger and swollen. In addition, the expression of auxin transporter genes PIN-FORMED were downregulated in ARs of transgenic plant. Taken together, these results suggest that PtoWUSa could be involved in AR development in poplar through regulating the polar auxin transport in ARs.

关键词:adventitious roots; auxin; poplar; PtoWUSa; transgenic plant