作者：Li Jianbo1,2, Zheng Guangshun1, Li Shaofeng1, Tang Zhimin1, Sun Jingshuang1, Xin Xuebing1
单位：1 Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 102300, China; 2 State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.
期刊：Journal of Plant Biology. 2020. doi: 10.1007/s12374-020-09248-4
基金：Fundamental Research Funds of Chinese Academy of Forestry (CAFYBB2016MA008, CAFYBB2017ZD005 and CAFYBB2019ZY003).
摘要：As a class of AP2 family transcription factors, C-repeat binding factor (CBF) gene family plays an important role in resisting low-temperature stress and improving cold tolerance of plants. Rosa chinensis is an important horticultural and ornamental plant, but little is known about the molecular characteristics of CBF genes in R. chinensis. In our study, six RcCBFs members were identified at R. chinensis whole genome, including one member RcCBF1 on Chromosome 1 and other five members RcCBF2-6 on Chromosome 7. The analysis of cis-acting elements indicated that there were various cis-acting elements related to stress, development, hormone, and light. The expression profiles showed that most of RcCBFs were mainly expressed in root, and the expression levels of RcCBFs were significantly induced by low-temperature stress, especially RcCBF6. To verify the function of RcCBF6, we generated its overexpressing transgenic lines in Arabidopsis thaliana. The RcCBF6-overexpressing plants exhibited higher tolerance to cold stress as evidenced by a better growth and higher antioxidative enzyme activities than the wild-type plants. Furthermore, the expression levels of some cold-response genes were upregulated in the transgenic plants, such as KIN1, RD29A, LTP3, and GOLS3. Our study contributes to a better understanding of RcCBF gene family and provides a foundation for the further functional research of RcCBFs.
关键词：CBF · Phylogenetic analysis · Transgene · Cold tolerance · Rosa chinensis
